The following are examples of experiments that our core team has performed
10. Wheel running
11. Germ-free mice
13. Body Composition
1. Thermogenesis: cold temperature challenge
Mice were housed at 30C, 23C, and 4C while monitoring both body weight and energy expenditure in the Promethion indirect calorimeter. Male C57Bl/6J mice from Jax ~10-14 weeks old. Data plotted in CalR.
Thermogenesis: beta-adrenergic agonist activation of mice housed at 30C. Performed in the CLAMS indirect calorimeter. Data plotted in CalR.
3. Pharmacologic induction of metabolic rate with thyroid hormone (T3). C57Bl/6J male mice were monitored in the Promethion indirect calorimeter. Data plotted in CalR. Study in press.
4. Analysis of diet-induced obesity experiments: male C57Bl/6J mice, 11 weeks of low-fat diet (LFD) or high-fat diet (HFD). Corrigan et al eLife. Experiments were performed in the CLAMS at UC Davis.
5. Optogenetic stimulation of food intake in mice expressing Channelrhodopsin-2 (ChR2) in Agrp neurons. Pilot experiment with Jon Resch and Brad Lowell in the Promethion indirect calorimeter. Blue shading represents light pulses. X-axis represents clock time (14:00 = 2pm). Mice had free access to food and were made hungry by activating this neuronal population. Food intake increased, metabolic rate (VO2) increased, body temperature increased, and increased physical activity in mice that would normally be sleeping.
6. Chemogenetic stimulation. Mice expressing DREADDs vs non DREADD controls. All mice were given CNO at hour 4. Plotted in CalR. Performed in the Promethion indirect calorimeter.
7. Induction of torpor. Upon entering into the dark photoperiod in the absence of food, torpor causes a precipitous drop in both body temperature (25C) and metabolic rate. Raw data from two mice as preliminary studies leading to Hrvatin et al. Nature 2020. C57Bl/6J female mice. Blue: a mouse with free access to food. Red: a fasted mouse. Performed in the CLAMS. Plotted in R & ggplot2.
8. Rates of 13C-glucose oxidation in vivo using stable isotope tracing. C57Bl/6 mice either fasted or were given free access to food for 4 hours prior to administration of 2.5 mg 13C-glucose. Plotted in R & ggplot2. Performed in the Promethion indirect calorimeter.
9. Continuous Glucose Monitoring. Example coming soon.
10. When mice are introduced to a running wheel, daily wheel running and energy expenditure both increase over time in female mice. The wheel was introduced to the mice on the first day. Performed in the CLAMS
11. Germ-free mice can be examined using isolator cages (bottom). With this approach, we can examine the metabolic profile of germ-free mice or mice colonized with specific microbes.
12. Thermal imaging (FLIR) of juvenile mice placed in a sterile 6-well culture dish.
13. Body Composition analysis performed on an EchoMRI 3-in-1 device and plotted in CalR. Raw data (in grams) are plotted for fat mass, lean mass, and total mass (left). The percent body composition is also plotted (right).